Title: a Rapid Dna Extraction Method Suitable for Human Papillomavirus (hpv) Detection

method suitable for human papillomavirus detection. Abstract: 33 Infection with oncogenic human papillomavirus (HPV) genotypes is necessary for the 34 development of cervical cancer. Testing for HPV DNA from liquid based cervical 35 samples can be used as an adjunct to traditional cytological screening. In addition 36 there are ongoing viral load, genotyping and prevalence studies. Therefore, a sensitive 37 DNA extraction method is needed to maximise the efficiency of HPV DNA detection. 38 The XytXtract Tissue kit is a DNA extraction kit that is rapid and so could be useful 39 for HPV testing, particularly in screening protocols. This study was undertaken to 40 determine the suitability of this method for HPV detection. DNA extraction from 41 HeLa and Caski cell lines containing HPV 18 and 16 respectively together with DNA 42 from five liquid based cervical samples were used in a HPV PCR assay. DNA was 43 also extracted using the QIAamp DNA mini kit (Qiagen, Hilden, Germany) as a 44 comparison. DNA extracts were serially diluted and assayed. HPV DNA was 45 successfully detected in cell lines and cervical samples using the XytXtract Tissue kit. 46 In addition, the XytXtract method was found to be more sensitive than the QIAmp 47 method as determined by a dilution series of the extracted DNA. While the XytXtract 48 method is a closed, the QIAamp method uses a spin column with possible loss of 49 DNA through DNA binding competition of the matrix, which could impact on the 50 final extraction efficiency. The XytXtract is a cheap, rapid and efficient method for 51 extracting HPV DNA from both cell lines and liquid based cervical samples. 52 53 Introduction 54